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Image Search Results
Journal: Oncotarget
Article Title: Icaritin inhibits the expression of alpha-fetoprotein in hepatitis B virus-infected hepatoma cell lines through post-transcriptional regulation
doi: 10.18632/oncotarget.13194
Figure Lengend Snippet: A. Schematic showing potential binding sites of predicted miRNAs in the AFP-3′-UTR construct. B. Effect of transfection of various miRNA mimics on the expression of AFP . The panel on the right side of images is the densitometric analysis. C. Homology analysis of miR-620, miR-1236 and miR-1270 in human, chimp and rhesus. D. Relative luciferase activity after transfection of the AFP-3′-UTR construct and miR-620, miR-1236, miR-1270 mimics or inhibitors. E. A schematic plot of the luciferase construct AFP-3′-UTR-MU expressing mutational binding sites of miR-620, miR-1236, miR-1270 (upper panel). Relative luciferase activity after co-transfection of AFP-3′-UTR-MU construct and miR-620, miR-1236, miR-1270 mimics (left panel) or inhibitors (right panel). Underlined letters in the schematic represent mutated nucleotides. F. Schematic plot of the luciferase constructs AFP-3′-UTR-MU 1 expressing mutational binding site of miR-1236 and AFP-3′-UTR-MU 2 expressing mutational binding sites of miR-620, miR-1270 (upper panel). Luciferase activity after co-transfection of AFP-3′-UTR-MU 1 or AFP-3′-UTR-MU 2 constructs and miR-620, miR-1236, miR-1270 mimics (left panel) or inhibitors (right panel). These experiments were repeated at least three times. Data represents mean ± SD of three samples. * P < 0.05 and ** P < 0.01 as compared with controls.
Article Snippet: Detection of miR-620, miR-1236 and miR-1270 was carried out by quantitative real-time PCR after reverse transcription using a
Techniques: Binding Assay, Construct, Transfection, Expressing, Luciferase, Activity Assay, Cotransfection
Journal: Oncotarget
Article Title: Icaritin inhibits the expression of alpha-fetoprotein in hepatitis B virus-infected hepatoma cell lines through post-transcriptional regulation
doi: 10.18632/oncotarget.13194
Figure Lengend Snippet: Schematic model of the possible role of miR-620, miR-1236 and miR-1270 in the post-transcription inhibition of AFP expression in hepatoma cells and the effects of icaritin and HBV on these miRNAs
Article Snippet: Detection of miR-620, miR-1236 and miR-1270 was carried out by quantitative real-time PCR after reverse transcription using a
Techniques: Inhibition, Expressing